Seminar

Seminar

Date:
6 February 2012 00:00 hrs.
Location:
Title:
Extensive Promoter-centered Chromatin Interactions Provide a Topological Basis for Transcription Regulation
Speaker(s):

Dr. Guoliang Li, Genome Institute of Singapore

Host(s):

Prof. Henk Stunnenberg, Department of Molecular Biology, NCMLS, Nijmegen

06-02-2012 00:00:00Europe/AmsterdamExtensive Promoter-centered Chromatin Interactions Provide a Topological Basis for Transcription Regulation Rimlsrimls@radboudumc.nl

Remarks / more information:

Higher-order chromosomal organization for transcription regulation is poorly understood in eukaryotes. Using genome-wide Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET), we mapped long-range chromatin interactions associated with RNA polymerase II in human cells and uncovered widespread promoter-centered intra-genic, extra-genic and inter-genic interactions. These interactions further aggregated into higher-order clusters, wherein proximal and distal genes were engaged through promoter-promoter interactions. Most genes with promoter-promoter interactions were active and transcribed cooperatively, and some interacting promoters could influence each other implying combinatorial complexity of transcriptional controls. Comparative analyses of different cell lines showed that cell-specific chromatin interactions could provide structural frameworks for cell-specific transcription, and suggested significant enrichment of enhancer-promoter interactions for cell-specific functions. Furthermore, genetically-identified disease-associated non-coding elements were found to be spatially engaged with corresponding genes through long-range interactions. Overall, our study provides insights into the transcription regulation by three-dimensional chromatin interactions for both housekeeping and cell-specific genes in human cells.

 

Collaboration with Xiaoan Ruan, Raymond K. Auerbach, Kuljeet Singh Sandhu, Michael Snyder, Yijun Ruan

 



<< back to all events