18 January 2012 00:00 hrs.
Protection against a malaria challenge by sporozoite inoculation; humoral correlates of protection.

Dr. Philip Felgner, Associate Professor,   Department of Medicine, Division of Infectious Diseases, University of California, USA


Prof. R. Sauerwein, Medical Microbiology, RUNMC

18-01-2012 00:00:00Europe/AmsterdamProtection against a malaria challenge by sporozoite inoculation; humoral correlates of protection.

Remarks / more information:

Philip Felgner has pioneered a high-throughput cloning and protein microarray chip fabrication approach that is useful for profiling immunoreactivity on a large scale. Using a special high-throughput recombination method developed his group has cloned more than 30,000 plasmids derived from 30 infectious microorganisms, expressed and printed the encoded proteins on microarrays.

 We recently induced protection against a malaria infection in volunteers through immunization by bites of infected mosquitoes while simultaneously preventing disease with chloroquine prophylaxis (CPS)*. This protective immunity was associated with high specific cellular responses.

To explore correlates of protection of humoral reactivity, a highly sensitive proteome microarray against 853 empirically selectedPlasmodium falciparumantigens was used.

Reactivity of naïve non-immunized infected volunteers was associated with elevated antibody titers against hundreds ofP. falciparumantigens and the response is enriched in certain blood-stage antigens. CPS immunized individuals who are protected from the sporozoite challenge also generate antibodies against dozens ofPfantigens. No reactivity was observed against blood stage antigens, however high reactivity was detected against pre-erythrocytic antigens which correlated with protection against malaria.

* : Roestenberg et al, N Engl J Med. 2009

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