Seminar

Seminar

Date:
8 December 2011 00:00 hrs.
Location:
Title:
Getting in and out of the nucleus – connecting nuclear pores and antigenic variation in African trypanosomes
Speaker(s):

Prof. Mark C. Field, Department of Pathology, University of Cambridge, UK

Host(s):

Dr. John van Dam, Centre for Molecular and Biomolecular Informatics, NCMLS, Nijmegen

08-12-2011 00:00:00Europe/AmsterdamGetting in and out of the nucleus – connecting nuclear pores and antigenic variation in African trypanosomes Rimlsrimls@radboudumc.nl

Remarks / more information:

Mark FieldA unifying feature of eukaryotic nuclear organization is genome segregation into transcriptionally active euchromatin and transcriptionally repressed heterochromatin. In metazoa, lamins preserve nuclear integrity and higher order heterochromatin organization at the nuclear periphery, but no lamin orthologues have been identified in non-metazoa. Moreover in yeast, lamins and a nuclear lamina are clearly absent, despite the presence of heterochromatin, implying that lamin-dependent heterochromatin organization in metazoa is a lineage-specific feature, and evolved relatively recently. We sought to determine if lamina-mediated heterochromatin organization and gene regulation at the nuclear periphery is restricted to metazoa or if this mechanism is also present in the divergent eukaryote, Trypanosoma brucei, and hence likely widespread. Further, taking a combination of biochemical, proteomic and comparative genomics approaches we examined the origins of the nuclear pore complex. Significantly we find that the NPC, and the transport machinery is highly conserved between taxa, but that the lamina is not, and appears to have rather distinct structures in different taxa. The functional and evolutionary consequences of these findings will be discussed.



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