Seminar: Ashley Toye

Investigating the requirement of ankyrin for membrane protein stability in differentiating human erythroblasts

Date:
2 June 2016 15:30 hrs. - 16:30 hrs.
Location:
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
Title:
Investigating the requirement of ankyrin for membrane protein stability in differentiating human erythroblasts
Speaker(s):

Ashley Toye, University of Bristol, UK

Host(s):

Merel Adjobo-Hermans, Dept. of Biochemistry, Radboudumc

02-06-2016 15:30:0002-06-2016 16:30:00Europe/AmsterdamInvestigating the requirement of ankyrin for membrane protein stability in differentiating human erythroblasts Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289Rimlsrimls@radboudumc.nl

Remarks / more information:

undefinedThe red blood cell has a specialised membrane and underlying cytoskeleton, which is essential for its role in the body as a vehicle for gas exchange. There are numerous naturally occurring genetic mutations that impact on red blood cell shape e.g. band 3, spectrin, ankyrin, and protein4.2. Although studying these mutations in the mature erythrocyte, and the secondary protein deficiencies that often also occur, has provided important information about protein hierarchy, they do not tell us how or when during erythropoiesis these deficiencies occur. The Toye lab uses patient samples and shRNA knockdowns (and is now moving to CRISPR) to probe the role of various proteins during membrane biogenesis to better understand the rules that make a red blood cell. 

This lecture will concentrate on recent work exploring the role of ankyrin in stabilizing proteins throughout erythropoiesis. 

Key Publications: 

  • The cytoskeletal binding domain of band 3 is required for multiprotein complex formation and retention during erythropoiesis. Haematologica, 2015  
  • Characteristic phenotypes associated with congenital dyserythropoietic anemia (type II) manifest at different stages of erythropoiesis. Haematologica, 2013 
  • Critical band 3 multiprotein complex interactions establish early during human erythropoiesis. Blood, 2011​


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