Seminar: Dr. Fuchou Tang

Dissecting gene regulation network in human early embryos at single-cell and single-base resolution

Date:
5 September 2014 00:00 hrs.
Location:
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
Title:
Dissecting gene regulation network in human early embryos at single-cell and single-base resolution
Speaker(s):

Dr. Fuchou Tang, Laboratory of Stem Cells and Epigenetics BIOPIC, College of Life Sciences, Peking University, China

Host(s):

Dr. Jo Zhou, Dept. of Molecular Developmental Biology, RIMLS

05-09-2014 00:00:00Europe/AmsterdamDissecting gene regulation network in human early embryos at single-cell and single-base resolution Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289Rimlsrimls@radboudumc.nl

Remarks / more information:

Fuchou TangMeasuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development. Here we apply single-cell RNA sequencing (RNA -Seq) analysis to 124 individual cells from human preimplantation embryos and human embryonic stem cells (hESCs) at different passages. We also systematically profile the methylome of human early embryos from the zygotic stage through to post-implantation by reduced representation bisulphite sequencing (RRBS) and whole-genome bisulphite sequencing (WGBS). We show that the major wave of genome-wide demethylation is complete at the 2-cell stage, contrary to previous observations in mice. Moreover, the demethylation of the paternal genome is much faster than that of the maternal genome, and by the end of the zygotic stage the genome-wide methylation level in male pronuclei is already lower than that in female pronuclei. Finally, we also show that long interspersed nuclear elements (LINEs) or short interspersed nuclear elements (SINEs) that are evolutionarily young are demethylated to a milder extent compared to older elements in the same family and have higher abundance of transcripts, indicating that early embryos tend to retain higher residual methylation at the evolutionarily younger and more active transposable elements. Our work provides insights of critical features of the transcriptome and DNA methylome landscapes of human early embryos, as well as the functional significance of DNA methylome to regulation of gene expression and repression of transposable elements.

Key Publications:

  • Guo H, Zhu P, Yan L, Li R, Hu B, Lian Y, Yan J, Ren X, Lin S, Li J, Jin X, Shi X, Liu P, Wang X, Wang W, Wei Y, Li X, Guo F, Wu X, Fan X, Yong J, Wen L, Xie SX, Tang Fuchou*, Qiao J*. The DNA methylation landscape of human early embryos. Nature 511: 606-610 (2014) (*: Co-corresponding author).
  • Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang Fuchou*, Xie XS*, Qiao J*. Genome Analyses of Single Human Oocytes. Cell 155:1492-1506 (2013) (*: Co-corresponding author).
  • Yan L, Yang M, Guo H, Yang L, Wu J, Li R, Liu P, Lian Y, Zheng X, Yan J, Huang J, Li M, Wu X, Wen L, Lao K, Li R*, Qiao J*, Tang Fuchou*. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells. Nature Structural & molecular Biology 20: 1131-1139 (2013) (*: co-corresponding author).

 

 

 

 

 



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