Seminar: Dr. Pim Huis in 't Veld

Characterization of a DNA exit gate in the human cohesion ring

Date:
16 December 2014 12:00 hrs. - 13:00 hrs.
Location:
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
Title:
Characterization of a DNA exit gate in the human cohesion ring
Speaker(s):

Dr. Pim Huis in 't Veld, IMP, Research Institute of Molecular Pathology, Vienna, Austria

Host(s):

Dr. Colin Logie, Dept. of Molecular Biology, RIMLS

16-12-2014 12:00:0016-12-2014 13:00:00Europe/AmsterdamCharacterization of a DNA exit gate in the human cohesion ring Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289Rimlsrimls@radboudumc.nl

Remarks / more information:

Huis In T Veld , PimChromosome segregation depends on sister chromatid cohesion mediated by cohesin. Cohesin's Smc1, Smc3 and Scc1 subunits form tripartite rings that are thought to open at distinct sites to allow entry and exit of DNA. However, direct evidence for the existence of open forms of cohesin is lacking. Here we show that cohesin's proposed DNA exit gate is formed by interactions between Scc1 and the coiled-coil region of Smc3. Mutation of this interface abolished cohesin's ability to stably associate with chromatin and to mediate cohesion. Electron microscopy revealed that weakening of the Smc3-Scc1 interface, as well as proteolytic cleavage of Scc1, resulted in opening of cohesin rings. These open forms may resemble intermediate states of cohesin normally generated by the release factor Wapl and the protease separase, respectively.

Key Publications:

  • Characterization of a DNA exit gate in the human cohesin ring. Science. 346: 968-72, 2014
  • Cohesin's ATPase activity couples cohesin loading onto DNA with Smc3 acetylation. Curr Biol. 24:2228-37, 2014
  • Aurora B and Cdk1 mediate Wapl activation and release of acetylated cohesin from chromosomes by phosphorylating Sororin. Proc Natl Acad Sci U S A. 110: 13404-9, 2013







 



 



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