Seminar: Dr. Thijn Brummelkamp

Haploid Genetic Screens in Human Cells Decipher a Gene Network Involved in Virus Entry and Hereditary Dystroglycanopathies

Date:
4 April 2013 00:00 hrs.
Location:
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
Title:
Haploid Genetic Screens in Human Cells Decipher a Gene Network Involved in Virus Entry and Hereditary Dystroglycanopathies
Speaker(s):

Dr. Thijn Brummelkamp, Netherlands Cancer Institute

Host(s):

Prof. Hans van Bokhoven, department of Human Genetics and Dr. Dirk Lefeber, department of Neurology, RUMC

 

04-04-2013 00:00:00Europe/AmsterdamHaploid Genetic Screens in Human Cells Decipher a Gene Network Involved in Virus Entry and Hereditary Dystroglycanopathies Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289Rimlsrimls@radboudumc.nl

Remarks / more information:

Brummelkamp, Thijn

Experimental genetics provides a powerful window on genetic networks underlying complex biological systems. To expand the genetic toolbox for genetics in human cells we have recently developed a new approach based on insertional mutagenesis in haploid or near-haploid human cells. We have shown that this platform enables the generation of null alleles for most human genes and can be used to pinpoint genes that are involved in phenotypes of interest. Combined with parallel sequencing approaches, this method generates high-density genetic overviews of genes required for nearly any selectable cell trait. Recently we have used this novel genetic model system to identify host factors required for virus entry. This identified genes underlying a the O-glycosylation of dystroglycan for virus entry and simultaneously revealed genes mutated in hereditary syndromes involving congenital muscular dystrophy and severe defects of the brain and eyes.

 

 

 



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