Seminar: Guido Jenster, theme Urological cancers

Exosomes as biomarker treasure chests for prostate cancer

Date:
19 May 2016 11:00 hrs. - 12:00 hrs.
Location:
Location see remarks
Title:
Exosomes as biomarker treasure chests for prostate cancer
Speaker(s):

Guido Jenster, Dept. of Urology, ErasmusMC, Rotterdam

Host(s):

Gerald Verhaegh, Dept. of Urology, Radboudumc

19-05-2016 11:00:0019-05-2016 12:00:00Europe/AmsterdamExosomes as biomarker treasure chests for prostate cancer Location see remarksRimlsrimls@radboudumc.nl

Remarks / more information:

Location: N. Tulpzaal, A-building, route 606

 

undefinedDuring our search for novel serum protein biomarkers for prostate cancer (PCa), we discovered a decade ago that many of the cancer-derived proteins are secreted through small vesicles. These extracellular vesicles (commonly referred to as exosomes) not only contain proteins from the host cell, but also RNAs that are potential novel PCa markers. Among these exosomal RNAs are also long noncoding transcripts that are uniquely expressed in PCa.

Using confocal microscopy, the uptake and intracellular processing of exosomes was studied. Target cells internalize exosomes via endocytosis and direct them to lysosomes for degradation. In order to detect and capture extracellular  vesicles, we developed several assays including a test to count (sub)populations of exosomes and measure exosomal membrane proteins such as CD9 and CD63. These sensitive assays can measure vesicles directly in cell medium, urine and serum. After correction for urinary PSA, the CD9 and CD63 immunoassays were significantly higher in men with PCa. For our ongoing research, the immunoassay forms the basis for testing different antibodies directed against exosome membrane markers to generate disease-specific detection assays.

Publications:

  • Immuno-based detection of extracellular vesicles in urine as diagnostic marker for prostate cancer. Int J Cancer, 137:2869-78, 2015
  • Novel long non-coding RNAs are specific diagnostic and prognostic markers for prostate cancer. Oncotarget, 6:4036-50, 2015
  • Exosomal secretion of cytoplasmic prostate cancer xenograft-derived proteins. Mol Cell Proteomics, 8:1192-205, 2009


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