Seminar: Hermans & Van Noort

The higher order structure of chromatin; Single-molecule experiments on model fibers and on captured native gene fragments

Date:
22 February 2017 13:00 hrs. - 14:00 hrs.
Location:
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
Title:
The higher order structure of chromatin; Single-molecule experiments on model fibers and on captured native gene fragments
Speaker(s):

Klaas Hermans & John van Noort, University of Leiden

Host(s):

Colin Logie, Dept. of Molecular Biology, RIMLS

22-02-2017 13:00:0022-02-2017 14:00:00Europe/AmsterdamThe higher order structure of chromatin; Single-molecule experiments on model fibers and on captured native gene fragments Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289Rimlsrimls@radboudumc.nl

Remarks / more information:

 

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undefinedThe folding of chromatin defines access to our genes and therefore plays a pivotal role in transcription regulation. However, the structure of chromatin fibers is poorly defined and heavily debated. We use single-molecule techniques to probe and manipulate the dynamics of nucleosomes in individual chromatin fibres. These novel methods were initially applied to synthetic, highly homogeneous reconstituted nucleosome arrays and yielded unprecedented insight in the structure and dynamics of chromatin. Unfortunately, synthetic chromatin lacks the complexity that provides functionality to our epi-genome. In the second part, we will present a recently developed method to purify specific chromatin fragments from yeast without crosslinking the fiber. We will show the first single-molecule force spectroscopy results on intact, native fibers and discuss to possibilities to extend this method to specific human chromatin loci, which would uniquely probe chromatin structure, composition and variations at the single-molecule level. 

Key Publications

  • Multiplexing genetic and nucleosome positioning codes: a computational approach. PloS one 11, e0156905, 2016  
  • Quantitative analysis of single-molecule force spectroscopy on folded chromatin fibers . Nucleic acids research 43, 3578-3590, 2016 
  • Histone H3 phosphorylation near the nucleosome dyad alters chromatin structure. Nucleic acids research 42, 4922-4933, 2014


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