Seminar: Joachim Goedhardt

Connecting heterotrimeric G-protein activity with cell morphology

28 January 2016 11:00 hrs. - 12:00 hrs.
Location see remarks
Connecting heterotrimeric G-protein activity with cell morphology

Joachim Goedhardt, Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands


Alessandra Cambi, Dept. of Cell Biology, Radboudumc

28-01-2016 11:00:0028-01-2016 12:00:00Europe/AmsterdamConnecting heterotrimeric G-protein activity with cell morphology Location see

Remarks / more information:

undefinedG-protein coupled receptors (GPCRs) represent a large class of cell-surface receptors that directly activate heterotrimeric G-proteins. It has become increasingly clear that the actin cytoskeleton can be remodeled through GPCR activation, although the molecular mechanisms and its temporal and spatial aspects are poorly resolved. We employ functional imaging of heterotrimeric G-protein activity and Rho GTPase activity to understand the connection between heterotrimeric G-protein activation and cell shape. In addition, we have developed synthetic approaches to locally activate Rho GTPases. Together, these approaches allow for a detailed spatiotemporal analysis of protein activities and a deeper understanding how GPCRs control the actin cytoskeleton.

Key Publications:

  • A Perspective on Studying G-Protein-Coupled Receptor Signaling with Resonance Energy Transfer Biosensors in Living Organisms. Molecular Pharmacology 88: 589–595, 2015
  • Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization. Scientific Reports 5: 14693, 2015
  • Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nature Communications 3: 751, 2012


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