Seminar: Dr. Jürgen Schiller

Applications of (MALDI-TOF) Mass Spectrometry and 31P NMR Spectroscopy in Lipid Research.

Date:
13 October 2014 11:00 hrs. - 12:00 hrs.
Location:
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
Title:
Applications of (MALDI-TOF) Mass Spectrometry and 31P NMR Spectroscopy in Lipid Research.
Speaker(s):

Dr. Jürgen Schiller, University of Leipzig, Medical Department, Institute of Medical Physics and Biophysics, Leipzig, Germany.

 

Host(s):

Prof. Roland Brock, Dept. of Biochemistry, Radboudumc.

13-10-2014 11:00:0013-10-2014 12:00:00Europe/AmsterdamApplications of (MALDI-TOF) Mass Spectrometry and 31P NMR Spectroscopy in Lipid Research. Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289Rimlsrimls@radboudumc.nl

Remarks / more information:

Juergen _schiller _hpAlthough matrix-assisted laser desorption and ionization (MALDI) mass spectrometry (MS) ‑ often but not exclusively coupled with a time-of-flight (TOF) mass analyzer ‑ is particularly established in the protein field, there is increasing evidence that MALDI MS is also very useful in lipid research: MALDI MS is fast, sensitive, tolerates sample impurities to a considerable extent and provides very simple mass spectra with (often) negligible fragmentation of the analyte. Additionally, MALDI mass spectrometers originally purchased for "proteomics" can be used also for lipids without the need of major system alterations.

Two rather recent developments will be highlighted: "Imaging" MS is nowadays widely established and significant interest is paid in this context to the analysis of lipids because lipids ionize particularly well and are, thus, more sensitively detectable in comparison to other biomolecules such as proteins. It will also be shown that MALDI MS can be very easily combined with thin-layer chromatography (TLC) allowing the direct "scanning" of the TLC plate and the detection of lipids with a higher sensitivity than common staining protocols.

 



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