Seminar: Prof. Anna Akhmanova

Microtubule dynamics: a tale of two ends

Date:
6 November 2014 11:00 hrs. - 12:00 hrs.
Location:
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
Title:
Microtubule dynamics: a tale of two ends
Speaker(s):

Prof. Anna Akhmanova, Cell Biology, Faculty of Science, Utrecht University, the Netherlands

 

Host(s):

Dr. Alessandra Cambi, Dept. of Tumor Immunology, RIMLS

06-11-2014 11:00:0006-11-2014 12:00:00Europe/AmsterdamMicrotubule dynamics: a tale of two ends Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289Rimlsrimls@radboudumc.nl

Remarks / more information:

Akhmanova , AnnaMicrotubules are cytoskeletal filaments that control multiple aspects of cell architecture. The organization of microtubule networks depends on microtubule dynamics, which is regulated at the two microtubule ends, the plus and the minus end. At the plus ends, a group of microtubule regulators collectively known as +TIPs controls microtubule polymerization and interactions with different cellular structures. Several +TIPs regulate rapid processive microtubule growth, and we have recently found that this process is essential for mesenchymal cell migration in 3D matrices. At the minus ends, the members of CAMSAP/Nezha/Patronin protein family act as powerful stabilizing factors. By combining live cell imaging and in vitro reconstitution of microtubule assembly from purified components with laser microsurgery, we found that CAMSAP2 and CAMSAP3 are specifically deposited on the microtubule lattices formed by minus-end polymerization. This process leads to the formation of CAMSAP-decorated microtubule stretches, which are stabilized from both ends and serve as sites of non-centrosomal microtubule outgrowth. The physiological importance of CAMSAP-mediated organization of microtubule arrays will be discussed.

Key Publications:

  • Microtubule minus-end stabilization by polymerization-driven CAMSAP deposition. Dev Cell 28, 295-309, 2014
  • CFEOM1-associated kinesin KIF21A is a cortical microtubule growth inhibitor. Dev Cell 27, 145-60, 2013
  • An EB1-binding motif acts as a microtubule tip localization signal. Cell 138, 366-76, 2009

 

 



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