Seminar: Shashank Shekhar, theme Nanomedicine

Ménage à trois – How capping protein and formins together control filopodial size and dynamics

Date:
26 April 2016 14:00 hrs. - 15:00 hrs.
Location:
Location see remarks
Title:
Ménage à trois – How capping protein and formins together control filopodial size and dynamics
Speaker(s):

Shashank Shekhar, Carlier Lab, Institute of Integrative Biology of the Cell, Paris, France

Host(s):

Alessandra Cambi, Dept. of Cell Biology, RIMLS

26-04-2016 14:00:0026-04-2016 15:00:00Europe/AmsterdamMénage à trois – How capping protein and formins together control filopodial size and dynamics Location see remarksRimlsrimls@radboudumc.nl

Remarks / more information:


Location:
 Knowledge Square 6th floor, RIMLS Building, Route 283

undefinedundefinedundefinedundefinedActin cytoskeleton is essential for mechanotranduction which requires polarized assembly of actin filaments. Filament barbed ends are the “hotspot” of actin assembly regulation and are targeted by many regulating proteins. While Formins bind barbed ends and processively accelerate filament elongation, Capping Protein (CP) blocks filament growth. Using in vitro microfluidics-assisted single filament and single molecule imaging, we have recently demonstrated how formins and capping protein simultaneously bind barbed ends in a ternary complex. The complex formation at the barbed ends is sufficient for a quantitative description of both spatial and temporal regulation of filopodia.

 

 

Key Publications

  • Formin and Capping Protein together embrace the actin filament in a “ménage à trois”. Nature Communications, 2015
  • Profilin interaction with actin filament barbed end controls dynamic instability, capping, branching and motility. Developmental Cell, 2016
  • Kinetic studies provide key insights into regulation of actin-based motility. Molecular Biology of the Cell, 2016


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