Symposium

Imgaing-XL: Advances in intravital microscopy

Date:
15 September 2017 12:00 hrs. - 14:30 hrs.
Location:
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
Title:
Imgaing-XL: Advances in intravital microscopy
Host(s):

Peter Friedl, Dept. of Cell Biology, RIMLS

15-09-2017 12:00:0015-09-2017 14:30:00Europe/AmsterdamImgaing-XL: Advances in intravital microscopy Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289Rimlsrimls@radboudumc.nl

Remarks / more information:


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12:00 Roberto Weigert, National Institutes of Health, USA

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Title:
Molecular mechanisms of membrane remodeling in live animals by intravital subcellular microscopy.

Abstract:
Membrane remodeling is essential to regulate a variety of events such as organelle biogenesis, cell migration, cell division, and membrane trafficking. This process requires continuous modifications of the composition and the properties of the lipid bilayer but also mechanical forces that are directly applied to the membranes by cytoplasmic protein complexes. Although membrane remodeling has been extensively investigated in reductionist model systems, such as individual cultured cells and cell-free systems, the molecular mechanism regulating this process in mammalian tissues in situ remains largely unknown. We have been using Intravital Subcellular Microscopy (iSMIC) in live mice to study the spatiotemporal coordination of actin cytoskeleton, cell signaling, and cellular metabolism, which control the remodeling of the plasma membrane under both physiological and pathological conditions. Specifically, here we describe the machinery controlling the assembly and activation of the actomyosin cytoskeleton during membrane trafficking and cell motility.

Key publications:

  • Kinetics of milk lipid droplet transport, growth, and secretion revealed by intravital imaging: lipid droplet release is intermittently stimulated by oxytocin. Mol Biol Cell. 28(7):935-946, 2017
  • Concerted Actions of Distinct Non-Muscle Myosin II Isoforms Drive Intracellular Membrane Remodeling in Live Animals. J. Cell Biol.216(7):1925-1936, 2017

13:00 Chris Xu, Cornell University, USA

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Title:
In vivo 3-photon imaging of mouse brain.

Abstract:
In this talk, the fundamental challenges of deep tissue, high-resolution optical imaging are discussed. New technologies for in vivo structural and functional imaging of mouse brain using long wavelength excitation and 3-photon microscopy will be presented [1-2]. We will discuss the requirements for imaging the dynamic neuronal activity at the cellular level over a large area and depth in awake and behaving animals. We will speculate on the possible future directions to further improve the imaging depth and speed in biological tissues.

Key publications: 

  • In Vivo Three-Photon Imaging of Activity of GCaMP6-labeled Neurons Deep in Intact Mouse Brain. Nature Methods, 14, 388–390, 2017.
  • In vivo three-photon microscopy of subcortical structures of an intact mouse brain. Nature Photon., 7, 205–209, 2013.

14:00 Drinks and snacks

 

 

 



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