MMD Materclass

MMD mini-symposium: Chemical Immunology

27 September 2017 14:00 hrs. - 18:00 hrs.
Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route 289
MMD mini-symposium: Chemical Immunology

Carl Figdor / Martijn Verdoes, Dept. of Tumor Immunology, RIMLS

27-09-2017 14:00:0027-09-2017 18:00:00Europe/AmsterdamMMD mini-symposium: Chemical Immunology Figdor Lecture Theatre, 8th floor RIMLS Building, Geert Grooteplein 26-28, route

Remarks / more information:

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​14:00-15:00: Sander van Kasteren, Leiden Institute of Chemistry
Chemical tools for studying antigen presentation.

undefinedThe activation of specific T-cells by antigen presenting cells (APC) is one of the the pivotal step in the activation of the adaptive immune response. This activation happens by the recognition of antigen-derived peptides on the APC presented in the context of MHC-molecules. The process resulting in the selection and presentation of specific peptides by APCs are complex and many aspects of the process remain a ‘black box’. One of the major complicating factors is that a hallmark of this process is proteolytic degradation. This makes the study of late events difficult as standard detecting groups do not survive this degradation; or they affect the degradation process itself. The van Kasteren-group focuses on developing new chemical methodology based on, for example, click chemistry to study this degradation pathway. The rationale behind this approach is that the small handles available for click chemistry are not degraded during proteolysis, nor do they affect the rate of proteolysis. Using this approach we aim to image the antigen throughout the whole processing pathway. For this they have developed new imaging approaches. They have also shown recently that these bioorthogonal ligations can be performed once peptides are loaded onto MHC.
Using this approach they wish to not only determine what factors determine which peptides end up presented to T-cells, but also look at the rates at which these events occur.

​15:00-16:00: Geert-Jan Boons, Utrecht University / University of Georgia
Immune modulation through protein glycosylation.

Protein glycosylation has been implicated in many aspects of adaptive immune activation. It can greatly influence uptake and proteolytic processing of antigens. Glycosylated peptides can be presented by MHC-I and MHC-II, and the resulting complex can be recognized by T-cells, leading to glycan-dependent T-cell and cytotoxic T-cell responses. Most of the receptors involved in adaptive immune activation are glycosylated and the interaction of these glycoproteins with glycan-binding proteins, such as C-type lectins, galectins and Siglecs, can regulate many aspects of immune cell activation.  A number of chemical approaches will be described that can unravel at a molecular level the involvement of complex glycans in immune activation. It will be shown that such information can be exploited for the rationale design of vaccines and immune modulators. 

16:00-17:00: Paul Parren, Leiden University
Complement activation: a mechanism of therapeutic antibodies.

undefinedMonomeric antibodies of the IgG isotype organize into ordered hexamers after binding their cognate antigen expressed on a cell-surface. This process is dependent on specific interactions between Fc domains of neighboring IgG molecules in the hexamer, and it represents the first step of the classical pathway of complement activation. While unraveling the molecular mechanisms of IgG hexamerization, we identified point mutations that either inhibited or stimulated the formation of IgG hexamers and complement-dependent cytotoxicity (CDC).
This lecture will provide an overview of our insights into how antibodies activate complement and put this in perspective of immune defense. Published data will be used to show how such insight may be translated into the development of improved and more potent biotherapeutics.

17:00-18:00 Drinks and informal discussions.


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