Novel technology to study chromatin cross-talk at genome-wide scale

Brinkman, Arjen

Arjen Brinkman (photo) and Henk Stunnenberg have teamed up with collaborators in the Broad Institute (Meissner laboratory, Boston, USA) to establish a novel technology called 'ChIP-BS-seq' (Genome Research, March 30th 2012) to decipher the relationships between DNA methylation and other chromatin features. When applied to mouse embryonic stem cells they found that the key repressive mark called H3K27me3 and DNA methylation are mutually exclusive on CpG islands. Removal of DNA methylation caused widespread H3K27me3 genomic changes, confirming the antagonism between the two marks. This new technology provides a unique way of investigating global interdependencies between DNA methylation and other chromatin features.


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